FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8754424
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA71550.029.438324145
GTACTAA33150.018.0952551
GTTCTAG43650.017.9493161
GTACTAG20000.017.750891
GGGGGGG430350.017.46116145
GTATAGG25650.015.9885681
GTACGCG6252.5465852E-1115.6697521
GTCCTAG37650.015.4447471
GTATTAG36600.015.3861121
GTCCTAA35250.015.10714151
GTACAGG61700.014.6824561
GTGCTAG35700.014.5738021
GTATTAA54700.014.3233571
GGTCTAG28950.014.1660381
TCTGTAC68800.014.1463143
CTGTACT75400.013.8823124
GTACATG61900.013.8439351
TCTATAC37950.013.71013453
GTCTTAG35400.013.6598521
TAGTACC23800.013.6312594
Produced by FastQC (version 0.11.5)