FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8754424
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA70650.021.691765145
GTACTAG21250.020.745041
GTATTAG36000.017.0074121
GTCCTAG36200.016.9134481
CTAGACT31650.016.625234
GTTCTAG44650.016.45511
GTGCTAG37150.015.82171
GTATTAA58450.015.3983271
CCTGTCG29000.015.193117145
GTCCTAA33800.015.0349551
GTATATA70200.014.5653221
GGTCTAG30000.014.2862251
GTTCTAA49400.014.1292341
GTCCTAT33650.014.0102661
GTACTAT33800.013.9480911
CTGTACT72300.013.455564
TGTACTG63100.013.3806685
TCTATAC37750.013.2907583
TATACTG49300.013.2789865
GTACAGG65950.013.18300151
Produced by FastQC (version 0.11.5)