FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8761023
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA72950.039.83362145
CGCGTAT7809.799378E-619.607708145
GGGGGGG453200.018.51851145
GTACTAA35400.018.4738481
GTACTAG20450.018.2311231
GTCCTAG35950.017.8511941
GTATTAA58400.017.2682361
GTATTAG39000.017.0820031
GTTCTAG45700.016.8512151
GTATAGG26100.016.6262661
GTCCTAA30500.015.8308461
GTGCTAG34900.015.2359911
GTTCTAA48300.014.4256251
GTATATA71250.013.8107711
GCTCTAG40750.013.7986661
GTATAGA43000.013.6451921
GTCTTAG35850.013.6388481
CTATACT38550.013.6348694
TATGCCG35250.013.55852145
GTGTATA49550.013.3216181
Produced by FastQC (version 0.11.5)