FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8761023
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA73500.024.656073145
GTCCTAG38100.019.4176881
TAGATCG9402.2011209E-618.45857145
GTATTAG37450.017.9588281
GTACTAG20300.017.7702331
CGCCGTA23000.017.602522145
TGACGCG12308.6332875E-817.241339145
GTGCTAG36900.015.5753781
GTTCTAG45350.015.2348531
GTATTAA62050.015.0760231
GTCCTAA35100.014.9805751
CCTGTCG29800.014.879727145
GTCTAGA37200.014.4636141
GTCTTAG39450.014.413621
GTACAGG62800.014.2144581
GTATATA73450.013.9847461
GGTCTAG29750.013.9752331
GTATAGG27150.013.9623641
GTTCTAA49950.013.9542491
GTACAGA74200.013.9257921
Produced by FastQC (version 0.11.5)