FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14716955
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17650.019.78941
GTCCTAG37350.018.0551031
GTATTAG26650.016.739731
GTTCTAG42200.015.89811
GTATATA50700.015.2108281
GTGCTAG36800.014.5659851
GTCCTAA32950.014.4837031
GTCTTAG32750.014.3609641
GTACTAA26350.014.0429741
GCTCTAG42250.013.6692821
GTATTAA42750.013.5094081
GGTCTAG26950.013.34536651
GTGTTAG37350.013.1478191
CTATACT34700.012.8316944
GTATAGG25650.012.8083211
TCTATAC30300.012.6520573
TATACTG44100.012.5183945
GTATAAG30400.012.3996341
GTACAGG65250.012.2429891
CTAGACT31300.012.1303164
Produced by FastQC (version 0.11.5)