FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14768463
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG35200.016.7599411
GTACTAG17000.015.6264691
GTACTAA28000.015.0321411
GTATTAG28150.014.8294821
GTTCTAG41000.013.8841441
GTCTTAG31550.013.7781281
GTATTAA43800.013.4691761
GTCCTAA30100.013.2956791
GTATAGG24500.013.0959161
GTGCTAG36050.012.82385351
GGTCTAG25950.012.7630031
GCTCTAG41350.012.6819811
TATACTG41600.012.5236845
GTATATA49800.012.4698771
GTCTTAA37150.012.4441441
TTATACT51000.012.3801644
GTATATG43600.012.3440351
GTACTAT28150.012.255771
CGAATCG7150.012.0635899
GCGTTAG6900.011.9999991
Produced by FastQC (version 0.11.5)