FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15225615
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17900.016.7795521
GTCCTAG38700.016.5926651
GTATTAG30200.015.6612981
GCGCTAG5400.015.3437541
GTGCTAG37850.015.1410621
GTCTTAG32150.014.9261551
GTACTAA30500.014.7149111
GTTCTAG45950.014.1249351
GCTCTAG44800.014.1022111
GTCCTAA33800.013.9932311
GTTCTAA46300.013.869031
GGTCTAA28700.013.5928551
GTATTAA46550.013.0529031
GTGTTAG36850.013.0224121
GTATAGG23900.013.0004611
GTATATA49000.012.541171
GTACAGG67900.012.1518461
GTATTGG38600.012.0742631
GTACTAT28450.011.8920841
CTAGGCC41400.011.8308354
Produced by FastQC (version 0.11.5)