FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15130905
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG36400.016.8715381
GTACTAG19300.016.088711
GTTCTAG41500.014.9644381
GTACTAA27500.014.8042141
GTATTAG28350.014.2386491
GTGCTAG34150.014.0430071
GTCTTAG33950.013.515991
GTATTAA44200.013.2697491
GTATATA47200.013.1572921
GGTCTAG27300.013.0169911
GTTCTAA42350.012.8718121
GCTCTAG41200.012.7286511
GTCTTAA42450.012.678941
TCTAGAC31650.012.6453363
CTAGACT32950.012.4608094
GTCCTAA32600.012.3823841
GTATTAT43950.012.3247121
TTATACT53100.012.0857364
GTACAGG66200.012.0389891
GTGCTAA36450.012.0210461
Produced by FastQC (version 0.11.5)