FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15130905
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG18200.017.6440371
GTCCTAG39450.016.8926091
GTATTAG31700.016.447661
GTACTAA30250.014.9531371
GTATTAA48250.014.3126181
GTCCTAA32300.014.1110011
GTGCTAG33900.014.0561311
GTATAGG25150.014.0038871
GTACAGG63800.013.9090951
CTATACT35500.013.7054354
GTTCTAA45100.013.627931
GCTCTAG44250.013.4215171
GTTCTAG40750.013.3032721
TATACTG43200.013.259395
GTCTTAG33050.012.9549761
TCTATAC30100.012.7265783
GCGCTAG5700.012.7212811
GTATATA49650.012.5876821
GTATTAT47150.012.449551
GTGCTAA36450.012.4096691
Produced by FastQC (version 0.11.5)