FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056256
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG37700.026.73105145
GTACTAG13450.022.1034221
CACGAGC104800.019.854692145
TGCCGTG42100.019.804245145
GTCCTAG26750.019.7877771
GTATTAG22500.019.6582681
CGTCTTA22250.019.550766145
TCTCGTT31500.018.41289145
GTGCTAG24900.018.3459051
GTACTAA21050.017.912211
CTAGACT20750.017.4710524
CTACATG84300.017.11465145
GTCTTAA31200.016.965481
GTTCTAG29700.016.8457571
GTCCTAA23650.016.5561941
CTGTACT52950.016.4316984
GTACAGA49100.016.392281
GCTCTAA31150.016.0616051
GTATATA38200.015.9446091
GTCTAGA24600.015.916831
Produced by FastQC (version 0.11.5)