FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7077080
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT9700.044.81821145
CGCCGTT15100.027.35098145
CCGTATA12650.025.774897145
GACGATA22550.025.06242145
GTACTAA20200.023.3604721
CGACGAA23800.023.137241145
GTCCTAG27250.022.6449851
GTACTAG13500.022.0480251
GTCCTAA24050.020.5264321
TTATACC52350.020.207432145
CTATACT26250.019.3261344
TACGCGG16050.018.960474145
GTTCTAG29750.018.7898521
CTGCCGC124950.018.672161145
GTGCTAA27250.018.6488111
GTGCTAG25600.018.4328731
GGTCTAG21550.018.1913991
CCTCTAG40650.017.1447181
GTCTATA21650.017.101411
GGTCTAA20500.016.998361
Produced by FastQC (version 0.11.5)