FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425749
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG2900.020.1799141
GTATTAG5750.016.7632641
GTCCTAG5350.016.0862431
GTATTAA6700.015.9277811
GTCTATA4650.015.5465751
GTATTAT7650.014.3998161
GTATTAC5400.014.024821
GTCCTAA5650.014.0135381
GTACATA6700.013.8725831
TGCACCG5000.013.7709595
GTACAAA8800.013.69158651
GTATATA7400.013.49070551
TAGGACC4609.094947E-1213.471594
GTCTTAG5400.013.3873291
GTTCTAG6450.013.3428531
TAGACGC1550.00822766813.3267355
TACGACC1550.00822766813.3267354
TCAGTAC6200.013.326463
GGCTTAG4651.0913936E-1113.3256351
GCTCTAG6500.013.2402151
Produced by FastQC (version 0.11.5)