FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425749
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA4850.018.5056341
GTATTAG4200.018.0819721
GTACTAG3153.6379788E-1217.5340351
GTATATG5850.016.5224531
GTCCTAG5250.016.4381581
TGTATCG1959.2075625E-615.88310510
GTTCTAA7000.015.780631
GTATTAA7450.015.2907951
GTCTTAG4800.014.3833881
GTCTAGC4101.8189894E-1114.3132231
GTATAAG4353.6379788E-1214.2841911
CAGTACT9600.013.9902474
GCTATAG3751.0586518E-913.8080521
CTACGCT2002.0135971E-413.7750124
GCCTTAG5300.013.6777871
GATCTAG4304.1836756E-1113.6474921
GTACTAA4651.0913936E-1113.3626311
GTACAAG7750.013.362631
GGTCTAA4406.002665E-1113.3373231
GGACTAA4457.2759576E-1113.1874641
Produced by FastQC (version 0.11.5)