FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407543
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG4550.017.3845921
GCTCTAG6050.015.91661551
GTACTAA4600.015.7003581
GTCCTAC4250.015.3749151
GTGTTAG6100.014.658571
GAACTAG5900.014.5725681
TTAGTAC4102.0008883E-1114.2606794
GTCCTAA5550.014.2522341
ATAGTAC4455.456968E-1213.911363
GTACTAT4751.8189894E-1213.7565031
GTTCTAA6300.013.6473261
TCTAGGC7100.013.5630313
GGCTAGA4406.366463E-1113.2875331
GTGTAGA7050.013.1711211
GCTTATA5003.6379788E-1213.068681
GTCCTAG5300.012.9778351
CCTATAC4551.1277734E-1012.8497483
ATTATAC7500.012.839673
TTAGACC4852.3646862E-1112.7645634
GGTCTAG4852.3646862E-1112.7637661
Produced by FastQC (version 0.11.5)