FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407543
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36020.1496131117907344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG900.005743426719.1624871
CTATACT5700.017.5025024
GTATTAA7350.017.363561
GTCTTAG5800.017.246241
TTGCGCG1200.001509547917.19466212
GTACTAG3257.2759576E-1216.9809131
GTATTAG3850.016.1263541
CTAGTAC2856.164555E-915.7026643
GTACTAT5000.015.1766921
GTCTTAA6750.014.8189911
ACTATAC4650.014.8064823
CGCTAGG1400.0041690414.7661892
GTACTAA4700.014.6776511
CGGTACC2605.9153535E-714.5544684
GTCCTAG5500.014.4241281
TAGCACG1450.00532434314.2321355
GTATAAG4900.014.0785641
TCTAGAC5150.014.0374093
CTAGCCT6250.013.7605884
GCCCTAG6050.013.6829671
Produced by FastQC (version 0.11.5)