FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453432
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG3900.019.4115681
GTATAGC3750.016.5174791
GTATAGG2907.799827E-915.4258071
TTATCGC1350.003304951615.29084510
GTGTACG2302.646144E-614.9614841
GTGCTAG5050.014.3096981
GTATTAG5150.014.0318381
GGTCTAG4202.910383E-1113.9284311
CCTATAC4202.910383E-1113.9284313
TACGACC1750.001157746413.7645674
TCTAGAC5400.013.3822163
TAGTCGG1800.001434106813.3794899
TTATACT8500.013.3597274
GTATATA7800.013.2351591
GTACAGG9650.013.1940151
CTATACT5300.012.985444
GTGTTAG5053.6379788E-1212.946871
GTACTAA4551.10958354E-1012.8570131
GTCTTAT7050.012.6907351
GCCTTAG4953.45608E-1112.5132431
Produced by FastQC (version 0.11.5)