FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6206215
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6500.019.0935421
GTATAGG9300.015.3074411
GTATTAA16750.015.2547191
GTATAAG11600.014.4750741
GTACTAA9850.014.08212951
TAGTGCG3908.276402E-1014.03823857
GTTCTAG15800.013.8616571
GTGCTAG14700.013.4090311
GTATATG17100.013.2347751
GCTCTAG16850.013.2145041
GTACATA15200.013.2080921
GTATTAG11650.013.159651
GCCCTAG14150.013.1563271
TCTAGAC12550.013.0884953
CCGAGCC87850.013.08744173
GTCTTAG12800.012.8328621
ACGAGAC18550.012.78955572
TCCGAGC99600.012.78945573
GTCCTAG13750.012.7426421
CTAAGCG4901.4370016E-1012.6640854
Produced by FastQC (version 0.11.5)