FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6043479
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94360.1561352327028852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG13050.017.3453831
CTAAGCG4900.017.1323074
GTACTAG6600.016.5951061
GTACTAT9300.016.4880391
GTACTAA9000.015.0093521
GTATTAG11800.014.85121251
GTCCTAG14400.014.1980341
GTTCTAA17550.014.1460271
GTTCTAG15650.013.7638641
GTATATG16800.013.2563291
GGTCTAA11300.013.2467131
ATTAGAC10850.013.1207463
GTCCTAA12000.013.0824751
TGACGCT94050.012.96295873
CTTAGAC11650.012.8464033
GTGCTAA15150.012.7722061
GTATAGA13450.012.7578731
GACGCTG87150.012.69087973
GTATTAA16700.012.6798531
GCCCTAG13600.012.6171621
Produced by FastQC (version 0.11.5)