FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6292585
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA9900.016.9826431
GTTCTAG17950.015.6786591
GTCCTAG16000.015.5335811
GTGCTAG13800.015.3614261
GTACTAG6800.015.0498351
CCGACGA22350.014.86374373
CTAGACT13900.014.7095614
GTATTAG12200.014.3801711
CGATCGT4101.1459633E-1014.24341638
GTATATA17900.013.8847661
GGTCTAG12450.013.7978431
GTTCTAA17800.013.7574341
TCTAGAC14450.013.6454223
GTACCGT5251.8189894E-1213.2115946
CCTAGAC13300.013.1780353
GTATAAT18150.013.0893881
GTATTAA16300.013.0053811
GTACTAT10750.012.919861
GCGCTAA2851.3033443E-512.8244221
GGTCTAA11850.012.6458551
Produced by FastQC (version 0.11.5)