FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6193262
Sequences flagged as poor quality0
Sequence length79
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82350.1329670858426464No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG2852.7957867E-916.6693251
GTCCTAG14650.016.4636381
GTACTAG7500.016.0794871
GTATAGG9750.015.3673411
GGTCTAG13600.015.3163561
GTGCTAG15350.014.760561
GTTCTAA18550.014.5783131
GTATTAA17550.014.367841
GACGAAT16700.014.20866173
GTACTAT10250.013.9046551
CCCTTAG16300.013.9002811
CGACGAA18650.013.50599473
GGTCTAA12050.013.3439721
GTGTTAG13750.013.2888321
GTATATA19600.013.2379821
GTCTTAG12450.013.2087781
CTAGACT11900.013.1928134
GTATTAG12900.013.0312961
GTCTATA11250.012.6686871
GCTCTAG16650.012.5106571
Produced by FastQC (version 0.11.5)