FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6769137
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG23600.019.9500661
GTCTTAG28800.018.7141231
GTTCTAG31500.017.8967251
GTACTAG13200.017.8341481
GTGCTAG25850.017.7342951
GTCCTAG25750.016.1190811
GTATTAA41750.014.5416041
GTCCTAA23500.014.4989831
GTACTAA21450.014.4406071
GTATAAG25550.014.3055261
GTACAGG47900.013.8385631
GGTCTAA21950.013.5471961
GTGTAGG39750.013.4030631
GTACATA32900.013.3691931
CCTGTCG23351.0913936E-1113.318315145
GGTCTAG23850.013.2472131
CTGTACT53100.013.0622694
CCCTTAG30000.013.0095431
CTAGACT23350.012.9958384
GTCTTAT34250.012.8422321
Produced by FastQC (version 0.11.5)