FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6802084
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA45850.024.051464145
GTGCTAG27050.018.9839461
GTCCTAG28200.017.9903811
GTATTAG24350.017.531771
GTACTAG13000.015.2293821
GTACAGG50400.015.2218271
GTATAGG21750.014.7917551
GTATACG6706.366463E-1114.7747731
TGTACCG13950.014.6372695
CGTACAC12400.014.4697683
GTATTAC26400.014.0612181
GTACCGT11800.013.6337366
GTACTAA21250.013.3928981
GTATTAA37300.013.2695681
TAGACTG30950.013.1948245
GTTCTAG30050.013.1768371
GCCTTAG26350.013.1487061
TAGGACT29650.013.1469014
GTCCTAA25100.013.0640491
CTGTACT51700.013.0449844
Produced by FastQC (version 0.11.5)