FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6802084
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG26650.017.1975381
GTACTAA21250.017.1958961
GTCTTAG25600.017.1771131
GTGCTAG27150.016.8808251
TGTACCG13750.016.6637745
GTATTAG26100.016.3734571
GTGTTAG29550.015.3002021
GTCCTAA26800.015.2524951
GTATAGG18400.014.8103951
GTACTAG13000.014.7689681
CTGTACC50200.014.4329964
GCTCTAG31150.014.3154911
GGTCTAG20800.014.2925491
CTGTCTA43550.014.167131145
CTAGACT24600.014.0970174
GTTCTAG29600.014.018931
TAGACTG30600.013.9637475
GTACAGG52400.013.5924691
TCTATAC26000.013.5761263
TATACTG32000.013.3528345
Produced by FastQC (version 0.11.5)