FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6745434
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA48450.027.24293145
GTATAGG19900.018.3482421
GTCCTAG25850.016.7584171
GTGCTAG25750.016.3428271
TGTACCG14600.016.107675
CTAGACT23450.015.57080654
GTACCGT13750.015.3032836
GTATTAA39200.015.1558631
GTTCTAG30100.015.0090041
GTACAGG49600.014.5982141
GTATTAG23150.014.4357151
TCTGTAC50000.014.2340933
GTATAGA25600.014.0211461
GTACATA30400.013.843021
GTACTAA20700.013.75254251
GGGGGGG337600.013.633879145
CTGTACT53650.013.6117584
CTATACT26000.013.5676144
TAGACTG28350.013.5344485
GTATAAG24950.013.394261
Produced by FastQC (version 0.11.5)