FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6745434
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA45100.019.042913145
GTCCTAG25150.018.2365511
GTACTAG15300.017.8242071
GTACTAA22050.016.8652061
GGTCTAG21400.016.5085931
GTATTAG23600.016.2827851
GTTCTAG31300.015.4454041
GTCTTAG27750.015.4111521
GTGCTAG25550.015.0400671
CTATACT25750.014.921154
GTATATA38100.014.6408591
CCTATAC19350.014.0915753
GTCTAGG24700.014.0520621
GTATATG34950.014.0097031
GCTCTAG31450.013.9922221
TCTATAC24500.013.9118363
CCTCTAG40800.013.8238891
GTCCTAT22650.013.6820381
GTCTAGC19950.013.6696931
GCCTTAG27100.013.2650141
Produced by FastQC (version 0.11.5)