FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6663164
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG26150.020.83521
CTGTCTA44300.020.244781145
GTACTAA21000.017.9844531
GTACTAG14200.017.8764881
GGTCTAG21700.016.5483591
GTATTAG25250.014.9573661
GTCCTAA22400.014.9256211
GTACATA29100.014.8933281
GTATAAG24900.014.4216631
GTCTTAG28150.014.0762911
GTACTAT22400.013.820021
GTATAGG19350.013.7585971
GTTCTAG27950.013.7339831
TCTAGGC31950.013.5647133
ACGCGTT7650.0074547813.527056145
GTTCTAT31600.013.5191281
GTCCTAT23700.013.3231991
TCTAGAC21950.013.2570453
GTACAGG48650.013.2354031
GTACTAC16550.013.0935291
Produced by FastQC (version 0.11.5)