FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041081
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG3301.6552804E-1015.6196991
GTCCTAA3553.092282E-1115.48771
TTTGCGC1808.3654435E-515.2733433
GTACTAG1808.368942E-515.2725941
GGTCTAG4151.8189894E-1214.904581
GTATATG6000.014.89077951
GTCCTAG4303.6379788E-1214.3846521
GTATTGG5300.014.2640271
GTATTAC4353.6379788E-1214.2193121
CTATACT4950.013.8848574
GTATTAA6250.013.7453341
GTCCTAT4355.4569682E-1113.429351
ACTATAC3355.2948963E-813.3356793
GAGCTAG5200.013.2166681
TGGATCG2355.426093E-513.1610715
GTTCTAG5500.013.1205471
GTGTATA4204.4019544E-1013.09079551
GTGTTAG3952.4647306E-913.0493681
GAATACG1850.00177852413.0023441
TCTAACC5300.012.9679333
Produced by FastQC (version 0.11.5)