FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041081
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCA750.002035170622.9184044
GTTCTAG5200.017.8876191
GTTACGA1300.00254702115.9001061
GTGCTAG4600.015.727281
TCCGGCG2150.002785670315.68732770
TCTAGAC4600.014.9548493
CTAGACT4400.014.8448744
GTATTAA6850.014.5847691
GTCTAGG4303.6379788E-1214.4210271
GGTCTAA3605.9117156E-1014.3542641
TTATACT6600.014.0635654
GAACTAG5150.014.0476671
CCCCTAG5400.014.0352791
GTACTAA3951.6007107E-1013.9545241
TAGAACC5200.013.8832654
AACCGGT1500.0067138113.74261956
GTATATG5800.013.66131
TAGTACC2801.3749905E-613.5054874
GTCCTAG4609.094947E-1213.4805251
GGTCAGA10250.013.4439931
Produced by FastQC (version 0.11.5)