FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019213
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAA1056.1591074E-419.650021
GTATTAG3850.016.0772911
GTCCTAG4950.015.9780481
GGGCTAG3001.231092E-814.9012671
GTACTAC3404.1818566E-914.1595741
TCTAGGC5250.013.7553563
GTTCTAG6000.013.7550151
GTGCTAG3801.36788E-913.5740281
GTACTAG2052.5126422E-413.4195261
GTATAAG4457.8216544E-1113.1368121
GTCTTAG4457.8216544E-1113.1368121
GGACCGA2103.0782662E-413.1013126
GTCTAAG3952.4392648E-913.0585591
TATGCAC4551.1277734E-1012.848413
GTCTAGA3751.6530976E-812.8380151
GTATTAA6200.012.7566681
CCTATAC3801.9887011E-812.6694073
GTACTGA6950.012.3696181
CTAGGAC4206.0772436E-912.2818724
GTCTAGC2802.031435E-512.2812641
Produced by FastQC (version 0.11.5)