FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061171
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGT750.002036211522.91644
GTACTAG2701.5825208E-1017.8420471
GTATAGC3750.016.5166381
GTTGCGA1250.001988822816.5002118
CTAAGCG1300.00258465415.8652024
GTCGTAT1350.003301346715.2931831
GTCCTAG4300.015.204271
CGTTAAG1605.876592E-415.0542281
GTATTAA6350.014.6308811
GTGCTAA3606.020855E-1014.3373581
TCTGTAC9550.014.0391583
GTATTAT5500.013.7638651
GGATAGG4001.9826984E-1013.7638651
TACACCG1500.006691606213.7495075
GTTCTAG6550.013.6587981
GTATAGA4801.8189894E-1213.6204911
GATCGGC2052.520181E-413.414803511
GTATATG6450.013.3370791
GTACTTA6200.013.3198691
CGTCGTG1550.0083096513.3066218
Produced by FastQC (version 0.11.5)