FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009059
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT1154.7473994E-520.91705538
GCGATCG1154.751043E-520.9149737
GTCTAGA3650.019.7669771
GTACTAG2303.6561687E-1019.4191111
ACCGATT1301.2217056E-418.4961818
CTAGACG1300.00259352615.8570264
GTGCTAG3950.015.6563041
GCTAGAC3301.6552804E-1015.6167673
GTACGAA1350.0033352215.269731
ACGGGTC1605.95434E-415.0281478
GTATATA5750.014.9377781
CCTATAC3001.2456439E-814.8879863
ACTAGAC2554.817848E-714.820623
GTATTAG3951.0913936E-1114.78650951
GTGTTAG4251.8189894E-1214.5511531
ACGCATC1500.004808388714.45219963
TCTATAC4600.014.1908893
CTAGACA5400.013.9972524
GGATAGG3455.1986717E-913.9419261
GTCTAAT3709.295036E-1013.9284691
Produced by FastQC (version 0.11.5)