FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009059
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29410.14638694035366806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG450.00471897530.5387953
CGTTAGA1351.617809E-417.8302751
GTCCTAA3950.017.4111181
CCCCTAG4000.017.193481
CCTAGAC4000.016.319173
TAGGCCG1300.002595192815.8554995
CTAGACT4600.015.6831564
GCTCTAG4700.015.3643861
GTGTTAG4300.015.1942371
TCAGTAC5550.014.8567113
GTCCTAG4400.014.8489141
GCCCTAG4251.8189894E-1214.5638891
GTCTTAG3606.0754246E-1014.32791
GTATATG5200.013.8870411
GTTCTAA5800.013.6362081
GTATTAA6150.013.41931
CCCTAGG6200.013.3110811
TCGCACT1550.00834454913.2981614
TCTACGC1550.00834454913.29816110
GGCTTAG4406.366463E-1113.2858711
Produced by FastQC (version 0.11.5)