FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5097169
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6400.015.3993361
GTACTAA10400.015.0922271
GGTCTAG9950.014.6742221
GTCCTAA10750.014.2612961
GTCTTAG13700.013.8548221
GTTCTAG14150.013.6721751
CTAGGGT8850.013.6112314
GTCCTAG11000.013.6053391
TCCGAGC88000.013.2725973
GTATAAG9750.013.1033291
GTCCTAT9550.012.99552251
CTCCGAG113200.012.9618873
TCTAGAC9900.012.9047933
GCCCTAA9450.012.7467761
CACGAGA22950.012.5641473
GTATTAG9350.012.4927061
GCTCTAG12450.012.3139711
CTAAGCG3608.806255E-712.1676154
CCGAGCC77600.012.13518473
ACGAGAC17200.012.09580573
Produced by FastQC (version 0.11.5)