FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5097169
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95730.18781013539084146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA9450.017.3858091
GGTCTAG9400.017.089881
GTATTAG10900.015.7429311
GTCCTAA10300.015.2421231
CGACGAA14350.015.00733473
CCGACGA19800.014.74783473
GCTCTAG15500.014.604081
GTCTTAG12350.014.19021
GTATATA17300.013.9287471
GTGCTAG14000.013.8217181
GTCTTAA14800.013.814671
CTGACGC105500.013.77000273
TGACGCT84650.013.75520773
GGTCTAA10000.013.5087751
GTATTAA15800.012.9403251
GTATAGG8200.012.9121451
GGTTTAG11600.012.9044681
GTCCTAG10200.012.8859531
CCCTTAG12000.012.778571
GGATTAG11450.012.4357891
Produced by FastQC (version 0.11.5)