FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5334518
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10600.018.2511
GTACTAG5700.017.9308071
GTCCTAG14250.015.8815721
GTGCTAG13000.014.3200161
GTTCTAG14750.014.1058581
ACGAGAC17100.014.087773
GTTCTAA16500.013.9371281
TCTATAC11800.013.9199943
GTATATG15500.013.894311
GTACTAT10250.013.8885671
TCCGAGC90700.013.56172373
ATTAGAC10950.013.3338153
GTCCTAA11800.013.3015771
GTCTTAG11900.013.1897991
GTCTAGA11600.012.9015691
CGAGCCC60300.012.771955573
TATACCG2607.956146E-512.6350715
GTACATA14500.012.5868971
CTATACT11600.012.5866614
GCCCTAG11050.012.5527251
Produced by FastQC (version 0.11.5)