FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5334518
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG12650.017.62641
GTACTAG6300.016.8260331
GTCTTAG12700.016.1179051
CGACGAA14950.015.62775373
GTATTAG11150.014.0967131
GACGAAT12500.014.01809473
TGACGCT88350.013.8419173
CCGACGA19650.013.74760173
GTGCTAA13550.013.4882311
GTCCTAT11000.013.2920381
CTAGCGT2253.3248705E-412.9831274
ACGAATA10700.012.62338273
GTTCTAG13650.012.586051
GCTCTAG14950.012.4696221
GTATAGA12750.012.3277131
ACGCTGC61850.012.27669273
CTATACT12050.012.1211764
GACGCTG78800.012.04494773
TCTATAC10050.011.9909143
ATTAGAC11000.011.9512743
Produced by FastQC (version 0.11.5)