FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5219831
Sequences flagged as poor quality0
Sequence length79
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82720.15847256357533415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4900.017.8968221
GTCCTAG13100.017.0145021
CGACGAA16750.016.12713273
GTATATA17400.015.9597121
GCGCTAG2301.4178422E-615.8866711
GTCTTAA15750.014.8477331
GTGCTAA12950.014.6721681
GTCTTAG12500.014.6157381
GTTCTAG15100.014.2769621
GTACTAA9250.013.8256971
TCTAGAC11700.013.73220353
GTTCTAA14400.013.7022521
CCGACGA21950.013.63702273
GGTCTAG9750.013.491451
GTCCTAT10600.013.4437211
GTATTAG10050.013.088721
GTCTAGG10800.012.8564351
CGAATAG8550.012.80841473
GGTCTAA10300.012.7710321
GTCTTAC15800.012.7193921
Produced by FastQC (version 0.11.5)