FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7251517
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC76300.031.347502145
CCGTATA10850.026.052439145
CGCCGTT11250.024.481869145
GTCCTAG29100.022.9712661
GTACTAA23600.022.4750631
GTTCTAG33800.021.0668431
GTATTAG26450.020.6027721
GTGCTAG30850.020.2550531
GTCTTAG30100.019.3113921
GTATAGG20000.019.2546631
CTGCCGC137100.019.031744145
TTACGTT19500.017.841038145
GTACTAT26800.017.6225451
GCCGACC24700.017.606289145
GTACTAG15750.017.530451
GACGACA29900.017.45319145
TTATACC56900.017.451061145
GTATTAA40850.016.7195991
GTATTAT41150.016.5977061
GCCGTAA11800.016.584229145
Produced by FastQC (version 0.11.5)