FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7170090
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16600.022.7101881
CACGAGC72750.019.832151145
GTCCTAG31550.019.7617131
GTCTTAG28800.019.6348511
GTATAGG20750.019.2163141
GTATTAG25100.019.0631961
GTACTAA26150.018.8522281
CGTCTTA15300.018.00702145
GTACAGA55100.017.367921
GTGCTAG30500.017.351961
CGTCTTG28100.017.028929145
GTATATA49100.016.9801621
CCTATAC22750.016.8911533
GGTCTAG23250.016.8382241
CTATACT29900.016.731924
CTCCGAC29700.016.599773145
CTAGACT25650.016.394894
GCTCTAG32900.016.3065221
GTCCATA28050.016.2829531
GCCCTAG27350.016.1695521
Produced by FastQC (version 0.11.5)