FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7170090
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT9800.028.099335145
CCGACGC90350.025.746332145
TTACGTT20150.025.174536145
GTATTAG24700.024.1159631
GTACTAA23000.024.0034391
GTTCTAG32600.023.174131
GTCTTAG27350.021.2481141
GACGACA31100.020.505049145
GTACTAG16400.020.375191
CCGTATA13250.019.689066145
GTCCTAG27800.019.5976581
TATACGG5707.2759576E-1219.0713835
GTATAGG23000.018.6342491
GGTCTAA22150.018.3654691
GTGCTAG29950.018.1908131
GTGTTAG31400.018.0448231
GTTCTAA36450.017.7370011
GTCCTAT26500.017.5437241
GTATATA47250.017.2188421
CTATACT29300.016.825254
Produced by FastQC (version 0.11.5)