FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6996821
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTT23800.025.283644145
CGCCGTT13550.025.147633145
CCGTATA16450.025.121613145
GTTCTAG32800.023.4510291
GTATTAG25050.022.5952281
GTACTAG14100.021.615241
CTATACT28600.020.5330944
GTCCTAA26500.020.263561
GCCGTAA11650.019.914186145
GTACTAA23100.019.7905771
GCCGACC29250.019.333357145
GTTCTAA36950.018.6568811
GTGCTAG30850.018.58241
GGTCTAA22950.018.338981
CTAGACT24600.018.272254
GTCCTAG27550.018.1742861
GTCTTAA35250.017.7039091
CCGACGC137350.017.26067145
GCCCTAG25650.016.9743731
GTATAGG18550.016.429911
Produced by FastQC (version 0.11.5)