FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6946638
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15400.022.6126731
CGCCGTT14800.022.042776145
GCCGACC29450.020.924213145
GTCCTAG28900.020.3338011
GTGCTAG27150.020.041161
GTATTAG24700.019.6792831
CCGTATA19750.019.454687145
TTACGTT26500.019.14995145
CTATACT26750.018.4301474
GGTCTAA23250.018.4102821
GTTCTAG33200.017.7002051
CCGACGC151850.017.282608145
CTGTACT56450.016.696414
GTATATG37500.016.6379031
GTGCAGG94700.016.5476061
GTACTAA23900.016.3918231
GCTCTAG35050.016.3519841
GTTCTAA36050.016.3008841
GTACAGG51200.016.2951851
GTATAGG20300.016.0822891
Produced by FastQC (version 0.11.5)