FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12216619
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC111250.034.718792145
CGCCGTT21550.025.892908145
GTCTTAG47400.020.3744621
GTACTAG26300.019.3265861
CCGTATA17300.019.268555145
CGTGTAT25950.019.268555145
GTTCTAG60750.019.1243421
GTACTAA38250.018.9837231
TTACGTT30450.018.800837145
GTCCTAG53700.018.7954771
GTATTAG45050.018.6971781
TTATACC82100.018.359362145
CTGCCGC218900.018.075249145
GGTCTAG43700.017.2808361
GTGCTAG51350.017.2517131
GGTCTAA39950.016.9035931
GCTCTAG60100.016.4315011
GTCCTAA44200.016.4282231
GTATTAA66150.016.3557051
GTACTAT38050.016.220981
Produced by FastQC (version 0.11.5)