FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12250835
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG58950.024.106762145
GTCCTAG53850.019.658791
TAGCGCG24150.019.514744145
GTACTAA39250.019.3972111
CGTCTTA34100.018.923532145
GTACTAG26900.018.8684141
GTGCTAG52250.018.7342831
GTATTAG43550.018.4809571
TCTCGTT51100.018.303553145
GTATTAA67300.017.5615231
GTTCTAG59200.017.3922711
GTACATG82000.016.9776291
GTACAGG88900.016.6386491
GTATATA78450.016.3594991
GGTCTAA39400.016.1948181
GTCTTAG50800.015.9861531
GTCCTAA42550.015.8479481
GCTCTAG61350.015.8372531
GTGTAGA64050.015.7356731
GGTCTAG39850.015.6480331
Produced by FastQC (version 0.11.5)