FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12250835
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC120500.031.566952145
CGCCGTT17600.026.34679145
CCGTATA18750.024.730856145
GTACTAG27400.020.660921
GTATTAG43400.020.2349051
GTCCTAG52450.020.2028641
GTTCTAG58450.019.4948861
GTACTAA39550.019.452031
GTATTAA65850.017.6347681
GTCTTAG46800.017.5241991
GTCCTAA45050.017.2383041
GTTCTAA62500.017.1864931
GGTCTAG40450.016.5072561
CTGCCGC223500.016.4358145
GCTCTAG62150.016.3490471
GCCGACC50000.016.229622145
GACGACA49150.016.215471145
TTATACC83600.016.120075145
TCTAGAC47800.016.076453
CGTGTAT26800.015.950623145
Produced by FastQC (version 0.11.5)