FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11869592
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG68100.021.079401145
GTACTAG24600.020.9281651
GTACTAA36550.019.8390351
GTATTAG42800.019.8221151
GTCCTAG49200.019.3069691
GTGCTAG50050.017.8200511
GTTCTAG56550.017.4387041
GTATAGA48050.017.3545111
GTATAGG33700.016.9982851
TATACTG59750.016.6235875
GTCTTAG48800.016.4934331
GTATTAA64850.016.4367241
GTACATA51800.016.378121
TAGCGCG48100.016.27867145
GTGCTAA48550.015.9809461
GGGGGGG4585550.015.96714145
GTACAGG89050.015.7156111
GTATATA74400.015.3989831
GTATTGG50100.015.1970571
TGCCGTG83050.015.189723145
Produced by FastQC (version 0.11.5)