FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9286016
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG38200.023.343948145
CGTCTTA22100.022.307415145
CACGAGC111650.021.882843145
CTAGATG124000.021.10659145
CTATACT32900.020.4966454
CTCCGAC39650.019.930393145
GTATTAG30100.018.791491
TAGATCG31200.018.357141145
GTATATA49550.017.708221
GTATTAA44750.017.5010411
GTTCTAG45400.017.2504751
GTTCTAA41450.017.1448881
GTATAGG23100.016.6378441
GTCTTAG34350.016.466491
GGGGGGG5095950.016.379322145
GTCTAGA33650.015.9470291
ACACATA108900.015.911187145
CCCCTAG37250.015.7685551
TCTAGAC34550.015.7405763
GTATAAG30600.015.640681
Produced by FastQC (version 0.11.5)