FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9334497
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA19950.022.895864145
CTAGATG134500.021.5624145
CACGAGC115450.020.473074145
GTATTAA45350.019.8268011
CTCCGAC43250.019.613682145
CGTCTTG38450.018.856585145
GTCCTAG43250.018.7776031
ACCGTAA24650.018.530323145
GTATAGG25150.018.163971
GTCCTAA34400.017.7063521
GTATAGA33850.017.1371881
GTATATA52550.016.5583381
ACACATA112700.016.469313145
TAGATCG33700.016.350954145
CTGTACT73250.016.3307254
GTGTAGG47950.016.1809271
GGGGGGG5067500.016.17756145
GTGCTAG34600.016.1370031
CTATACG5402.8641807E-816.1107834
GTACAGA68550.015.9726771
Produced by FastQC (version 0.11.5)