FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9334497
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT10350.051.104504145
CCGTATA15100.035.02858145
CGCCGTT14600.033.746807145
GTACTAG18650.026.075861
GACGATA27500.024.50343145
GTCCTAG36200.022.657531
GTATTAG32450.022.3680441
CGTGTAT21850.021.222921145
GCCGACC35550.020.789173145
GTCTTAG32600.020.038611
TTATACC69900.020.005838145
CTGCCGC162200.019.699915145
CGACGAA27850.018.992157145
GTTCTAG41750.018.9501531
GTATAGG24500.018.0720081
GGTCTAA29400.018.0226331
GCCGTAA14350.017.672289145
GGTCTAG28300.017.440751
GTATTAA46000.017.1993241
TACGCGG18550.017.18642145
Produced by FastQC (version 0.11.5)