FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9079729
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29550.021.5946271
ACCGTAA31850.021.169373145
CGTCTTG44650.021.108448145
GTCTTAG32300.018.6335721
GTTCTAA39850.018.5606161
TGCCGTG52350.018.419142145
CGTCTTA32000.017.67173145
GTCCTAA30950.017.5719991
CTCCGAC44250.017.53093145
GCTCTAG42000.017.4378381
CTATACT33950.017.2979264
TGCCGTA28750.016.895514145
GTATTAA48350.016.7974011
GTGCTAG36300.016.7800481
CCTATAC24700.016.7311843
GCCCTAG31500.016.574581
GGGGGGG4676200.016.569033145
CTCTAGG59950.016.4476282
GGTCTAG30100.016.3818511
GTATATG43600.016.1326521
Produced by FastQC (version 0.11.5)